Salivary Desmoglein Enzyme-Linked Immunosorbent Assay for Diagnosis of Pemphigus Vulgaris: A Noninvasive Alternative Test to Serum Assessment

Table 1

Salivary anti-Dsg 1 and anti-Dsg 3 ELISA tests main diagnostic outcomes using three different cut-off values.

	Salivary anti-Dsg 1 ELISA			Salivary anti-Dsg 3 ELISA
Cut-off (RU/mL)	5.4	7.7	20	8.9	13.4	20
True positive	48	45	31	69	67	63
True negative	174	178	178	163	176	178
False positive	6	2	2	17	4	2
False negative	38	41	55	17	19	23
Sensitivity	55.8%	52.3%	36.1%	80.2%	77.9%	73.3%
Specificity	96.7%	98.9%	98.9%	90.6%	97.8%	98.9%
Positive predictive values	0.89	0.96	0.94	0.80	0.94	0.97
Negative predictive values	0.82	0.81	0.76	0.91	0.90	0.89
Test accuracy	0.83	0.84	0.79	0.87	0.91	0.91
Positive likelihood ratio (+LR)	16.7	47.1	32.4	8.5	35.1	65.9
Negative likelihood ratio (−LR)	0.5	0.5	0.6	0.2	0.2	0.3
Diagnostic odds ratio	36.6	97.7	50.2	38.9	155.2	243.8
Cohen’s kappa coefficient	0.82	0.83	0.78	0.86	0.91	0.90