High LIN28A Expressing Ovarian Cancer Cells Secrete Exosomes That Induce Invasion and Migration in HEK293 Cells

Figure 6

Relative levels of LIN28A and LIN28B mRNA and protein in HEK293 cells exposed to IGROV1 cell-secreted exosomes. HEK293 cells treated with vesicle-deplete IGROV1 conditioned media (vehicle), HEK293 cells treated with supernatant from exosome pellet (supernatant), and HEK293 cells treated with exosomes (exosome transfer). qPCR was performed to assess mRNA levels of LIN28A (a) and LIN28B (c) after HEK293 cells were exposed to IGROV1 cell-secreted exosomes. Data were normalized against the geometric mean of GAPDH, MRPS15, and TBP. Means without the same superscript are significantly different ( value <0.05). Western blot analysis was performed to determine LIN28A (b) and LIN28B (d) protein levels after HEK293 cells were exposed to IGROV1 cell-secreted exosomes. Densitometry of bands was determined to calculate relative protein amount. Means without the same superscript are significantly different ( value <0.05).

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