Evaluation of Antioxidant and Antibacterial Potentials of Nigella sativa L. Suspension Cultures under Elicitation

Table 2

TLC screening of thymoquinone and thymol production under different concentrations of pectin and MnCl₂ elicitation.

S. number	Color of spot	value	Different concentrations of pectin and MnCl2
			A	B	C	D	E	F	G	H
TLC for thymol
1	Thymol std. pink	0.76	+	+++	+	+	++	+	+++	−
2	Purple	0.97	−	+	−	+	+	+	+	+
3	Light blue	0.73	+	++	++	+	+	+	+	−
4	Light blue	0.60	++	++	−	+	++	−	++	−
5	Pinkish purple	0.43	+	+	+	+	−	+	−	−
TLC for thymoquinone
1	Thymoquinone std. yellow	0.82	++	+++	+	+	++	+	+++	−
2	Brownish yellow	0.95	+	+	+	+	−	+	+	+
3	Brownish yellow	0.68	+	++	−	+	+	++	+	+

A = epicotyl extract; B = control suspension culture; C = pectin 5 mg/L elicitation; D = pectin 10 mg/L elicitation; E = pectin 15 mg/L elicitation; F = MnCl₂ 5 mg/L elicitation; G = MnCl₂ 10 mg/L elicitation; H = MnCl₂ 15 mg/L elicitation; “−” = absent; and “+” = present.