Cyclophilin A Interacts with Viral VP4 and Inhibits the Replication of Infectious Bursal Disease Virus

Table 1

Primers and siRNAs used in this study.

Primers/siRNAs	Sequences (5′-3′)	Restriction enzyme	Usage
VP4-U	AGAGAATTCGCCGACAAGGGGTACGAG	EcoR I	pGB-VP4
VP4-L	AAACTGCAGAGCCATGGCAAGGTGGTA	Pst I
HA-VP4-U	CTGAATTCGCCGACAAGGGGTACGAG	EcoR I	pCAH-VP4
HA-VP4-L	GCCTCGAGAGCCATGGCAAGGTGGTA	Xho I
CypA-U	AAAGAATTCATGGCCAACCCCGTCGTG	EcoR I	pCAF-CypA
CypA-L	ATACTCGAGCCGAGAGCTGCCCGCAGTT	Xho I
pGEX-CYPA-U	GGCGAATTCGCCAACCCCGTCGTGTTC	EcoR I	pGEX-CypA
pGEX-CYPA-L	GCTGAGCTCCGAGAGCTGCCCGCAGTT	Xho I
T7SPU	TAATACGACTCACTATAGGGC		pGADT7
3ASPL	ACACGTAGCACGTGGTAGA
FVP5	CCTTCTGATGCCAACAAC		Real-time RT-PCR
RVP5	ACAATTAGCCCTGACCCT
PVP5	FAM-CGGACGACACCCTGGAGAAGCA-BHQ1
RNA#1	Sense, 5′-CCGAGUGGUUGGACGGCAATT-3′		siRNAs
	Antisense, 5′-UUGCCGUCCAACCACUCGGTT-3′
RNA#2	Sense, 5′-ACGGCAAGACGAGCAAGCATT-3′
	Antisense, 5′-UGCUUGCUCGUCUUGCCGUTT-3′
RNA#3	Sense, 5′-GACGAGAACUUCAUCCUGATT-3′
	Antisense, 5′-UCAGGAUGAAGUUCUCGUCTT-3′
Negative control (NC)	Sense, 5′-UUCUCCGAACGUGUCACGUTT-3′
	Antisense, 5′-ACGUGACACGUUCGGAGAATT-3′