Research Article
Cyclophilin A Interacts with Viral VP4 and Inhibits the Replication of Infectious Bursal Disease Virus
Table 1
Primers and siRNAs used in this study.
| Primers/siRNAs | Sequences (5′-3′) | Restriction enzyme | Usage |
| VP4-U | AGAGAATTCGCCGACAAGGGGTACGAG | EcoR I | pGB-VP4 | VP4-L | AAACTGCAGAGCCATGGCAAGGTGGTA | Pst I | | HA-VP4-U | CTGAATTCGCCGACAAGGGGTACGAG | EcoR I | pCAH-VP4 | HA-VP4-L | GCCTCGAGAGCCATGGCAAGGTGGTA | Xho I | | CypA-U | AAAGAATTCATGGCCAACCCCGTCGTG | EcoR I | pCAF-CypA | CypA-L | ATACTCGAGCCGAGAGCTGCCCGCAGTT | Xho I | | pGEX-CYPA-U | GGCGAATTCGCCAACCCCGTCGTGTTC | EcoR I | pGEX-CypA | pGEX-CYPA-L | GCTGAGCTCCGAGAGCTGCCCGCAGTT | Xho I | | T7SPU | TAATACGACTCACTATAGGGC | | pGADT7 | 3ASPL | ACACGTAGCACGTGGTAGA | | | FVP5 | CCTTCTGATGCCAACAAC | | Real-time RT-PCR | RVP5 | ACAATTAGCCCTGACCCT | | | PVP5 | FAM-CGGACGACACCCTGGAGAAGCA-BHQ1 | | | RNA#1 | Sense, 5′-CCGAGUGGUUGGACGGCAATT-3′ | | siRNAs | Antisense, 5′-UUGCCGUCCAACCACUCGGTT-3′ | | | RNA#2 | Sense, 5′-ACGGCAAGACGAGCAAGCATT-3′ | | | Antisense, 5′-UGCUUGCUCGUCUUGCCGUTT-3′ | | | RNA#3 | Sense, 5′-GACGAGAACUUCAUCCUGATT-3′ | | | Antisense, 5′-UCAGGAUGAAGUUCUCGUCTT-3′ | | | Negative control (NC) | Sense, 5′-UUCUCCGAACGUGUCACGUTT-3′ | | | Antisense, 5′-ACGUGACACGUUCGGAGAATT-3′ | | |
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