BioMed Research International

Research Article

An Activity of Thioacyl Derivatives of 4-Aminoquinolinium Salts towards Biofilm Producing and Planktonic Forms of Coagulase-Negative Staphylococci

Table 1

Phenotypic and genotypic characterization data from 41 CoNS strains.


Strain number	Bacterial species	Biofilm status	MP ( ± SD)	CRA plate	Presence of icaA/icaD

1	S. saprophyticus	Biofilm-negative	0.07 ± 0.01	r	ND
2	S. klosii	Biofilm-positive	0.44 ± 0.08	b	ND
4	S. cohnii subsp. urealyticum	Biofilm-negative	0.11 ± 0.01	r	ND
5	S. haemolyticus	Biofilm-negative	0.08 ± 0.02	r	ND
14	S. cohnii subsp. cohnii	Biofilm-negative	0.10 ± 0.04	g	ND
24	S. epidermidis	Biofilm-positive	0.48 ± 0.06	r	+/−
25	S. epidermidis	Biofilm-positive	0.44 ± 0.10	r	+/−
26	S. epidermidis	Biofilm-positive	0.61 ± 0.03	r	+/+
27	S. epidermidis	Biofilm-negative	0.07 ± 0.02	r	−/+
28	S. epidermidis	Biofilm-negative	0.08 ± 0.01	r	−/−
32	S. warneri	Biofilm-negative	0.06 ± 0.01	r	ND
33	S. epidermidis	Biofilm-positive	0.19 ± 0.01	r	+/−
53	S. epidermidis	Biofilm-negative	0.13 ± 0.01	r	+/+
62	S. cohnii subsp. cohnii	Biofilm-negative	0.07 ± 0.02	g	ND
64	S. epidermidis	Biofilm-negative	0.08 ± 0.02	r	−/−
65	S. epidermidis	Biofilm-positive	0.27 ± 0.08	r	−/−
76	S. epidermidis	Biofilm-positive	0.47 ± 0.05	r	+/−
78	S. epidermidis	Biofilm-positive	0.53 ± 0.10	r	+/−
84	S. epidermidis	Biofilm-negative	0.09 ± 0.02	r	−/−
90	S. epidermidis	Biofilm-negative	0.07 ± 0.01	g	−/−
91	S. epidermidis	Biofilm-positive	0.45 ± 0.04	b	+/+
93	S. haemolyticus	Biofilm-negative	0.05 ± 0.00	r	ND
95	S. epidermidis	Biofilm-negative	0.10 ± 0.05	r	−/−
96	S. haemolyticus	Biofilm-negative	0.11 ± 0.02	r	ND
97	S. epidermidis	Biofilm-positive	0.46 ± 0.01	r	+/+
99	S. epidermidis	Biofilm-positive	0.55 ± 0.05	r	+/+
101	S. haemolyticus	Biofilm-negative	0.06 ± 0.01	r	ND
102	S. epidermidis	Biofilm-negative	0.06 ± 0.00	r	−/+
103	S. capitis subsp. capitis	Biofilm-negative	0.14 ± 0.03	r	ND
105	S. haemolyticus	Biofilm-negative	0.10 ± 0.03	r	ND
107	S. epidermidis	Biofilm-negative	0.07 ± 0.02	r	−/−
125	S. warneri	Biofilm-negative	0.08 ± 0.03	r	ND
139	S. warneri	Biofilm-negative	0.10 ± 0.04	r	ND
12228	S. epidermidis ATCC 12228	Biofilm-negative	0.11 ± 0.02	r	−/−
35984	S. epidermidis ATCC 35984	Biofilm-positive	0.56 ± 0.11	b	+/+
15305	S. saprophyticus ATCC 15305	Biofilm-negative	0.09 ± 0.03	r	ND
27844	S. hominis ATCC 27844	Biofilm-negative	0.06 ± 0.01	r	ND
29970	S. haemolyticus ATCC 29970	Biofilm-negative	0.08 ± 0.03	r	ND
35661	S. capitis subsp. capitis ATCC 35661	Biofilm-negative	0.08 ± 0.01	r	ND
49454	S. warneri ATCC 49454	Biofilm-negative	0.08 ± 0.02	r	ND
49576	S. lugdunensis ATCC 49576	Biofilm-negative	0.06 ± 0.01	r	ND

CRA: type of growth on Congo Red Agar medium, r: red colonies, b: black colonies, and g: grey colonies;
icaA/D: (+) presence of the gene, (−): absence of the gene, and ND: not detection.
Correlation between biofilm status and MP test ; CRA plate test (); ica status (). Results were considered significant when ; Mann-Whitney nonparametric test; ²Rang Spearman test.