β-Catenin-Dependent Signaling Pathway Contributes to Renal Fibrosis in Hypertensive Rats

Figure 7

Inactivation of β-catenin signaling pathway in 2K1C rats by treatment with pyrvinium pamoate. The analysis of the status of different components of the Wnt signaling pathways by Western blot in the unclipped kidney from 2K1C Goldblatt rats treated with pyrvinium pamoate was performed. The levels of (a) β-catenin, (b) p-Ser9-GSK-3β, and (c) the target genes cyclin D1 and bcl-2 decrease after the treatment with pyrvinium pamoate in total extracts from unclipped kidney, indicating that the signaling is inactive. The level of protein was normalized to β-actin levels and the ratio was expressed as relative units normalized to sham rats. Numbers (1, 2, 3, and 4) in the Western blot indicate an individual animal sample in a given group. The bars represent the mean ± SEM (). .

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