Expression of Translationally Controlled Tumor Protein in Human Kidney and in Renal Cell Carcinoma

Figure 2

Western blotting analysis of TCTP protein expression in normal kidney and neoplastic specimens. WB was performed to confirm TCTP protein expression in normal kidney and in different renal tumors. A specific band of the MW of 22 kDa was detected in all the neoplastic and nonneoplastic specimens. Placenta was used as positive control. Relative expression of TCTP was measured with respect to actin, used as control. WB was carried out in triplicate. Densitometric analysis revealed higher expression of TCTP in tumor samples, with respect to normal kidney. (1) Normal kidney; ((2)–(5)) tumor samples: (2) ChRCC; (3) PRCC; (4) CDC; (5) CCRCC (WB: western blotting; MW: molecular weight; ChRCC: chromophobe renal cell carcinoma; PRCC: papillary renal cell carcinoma; CDC: collecting duct carcinoma; CCRCC: clear cell renal cell carcinoma).