Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts

Figure 5

Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) Four genes commonly downregulated and (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first and the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log ; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student’s -test; ) are indicated with an asterisk: , , and . (c) The blots show the protein expression levels in MRC-5 and HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots.

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