Advanced Glycation End Products Enhance Macrophages Polarization into M1 Phenotype through Activating RAGE/NF-κB Pathway

Figure 5

Role of AGEs on the expression of RAGE. For mRNA evaluation, the cells were cultured with different concentrations of AGEs for 24 h. For protein expression detection, the cells were incubated with AGEs 500 μg/mL for 24 h. BSA 500 μg/mL was used as a control. The mRNA levels were assayed by real time PCR and protein expression was assayed by FCM. AGEs upregulated RAGE mRNA expression in a dose dependent manner (a). AGEs significantly increased RAGE protein expression (b). , and compared with control. MFI: mean fluorescence intensity. Each bar represents the mean value ± SD of triplicate determinations, representative of three independent experiments.

(a)

(b)