Nordihydroguaiaretic Acid Disrupts the Antioxidant Ability of Helicobacter pylori through the Repression of SodB Activity In Vitro

Figure 3

The intracellular Fe²⁺ levels and the SOD activities of Mtz-resistant strains under exposure to 50 μM NDGA. (a) The intracellular Fe²⁺ levels of control strain (ATCC700392 pHel3 ctrl.), SodB-overexpressing strain (ATCC700392 pHel3::sodB), KS0048, fecA1-deletion mutant of KS0048 (KS0048ΔfecA1), KS0145, and fecA1-deletion mutant of KS0145 (KS0145ΔfecA1) were measured by the method described under Materials and Methods. Results are means ± SD of three independent assays. ; (Tukey test). (b) The SOD activities of control strain (ATCC700392 pHel3 ctrl.), SodB-overexpressing strain (ATCC700392 pHel3::sodB), KS0048, fecA1-deletion mutant of KS0048 (KS0048ΔfecA1), KS0145, and fecA1-deletion mutant of KS0145 (KS0145ΔfecA1) were measured by the method described under Materials and Methods. Results are means ± SD of three independent assays. ; (Tukey test).

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