Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells

Figure 3

Inhibition of miR-221 suppressed migration and invasion of UM1 cells. (a) Representative images of UM1 cell migration are shown. The migration of UM1 cells was measured using a transwell assay at 48 h after transfection with miR-221 inhibitor or miR-NC. (b) The average number of migrating cells per field for the indicated experimental groups is shown. (c) Representative images of UM1 migration cells analyzed by wound healing assays. Images show migration of cells after 0 h and 24 h. (d) Quantification of migrated UM1 cells analyzed by wound healing assays. The migration of UM1 cells transfected with miR-NC set to 100%. (e) Representative images of UM1 cell invasion are shown. The invasion of UM1 cells was measured using a Matrigel invasion assay at 48 h after transfection with miR-221 inhibitor or miR-NC. (f) The average number of invading cells per field for the indicated experimental groups is shown. Data are presented as means ± SD. ; .

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