Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells

Figure 5

The effect of MBD2 silencing on cell migration and invasion of UM1 cells after miR-221 transfection. (a) MBD2 mRNA expression 48 h after transfection with si-MBD2 or si-NC. (b) Western blot of MBD2 protein expression 48 h after transfection with si-MBD2 or si-NC. (c) Representative images of UM1 cell migration are shown. The migration of UM1 cells was measured using a transwell assay at 48 h after the transfection of miR-221 inhibitor plus si-NC or miR-221 inhibitor mimic plus si-MBD2. (d) The average number of migrating cells per field among the indicated experimental groups is shown. (c) Representative images of UM1 migration cells analyzed by wound healing assays. Images show migration of cells after 0 h and 24 h. (d) Quantification of migrated UM1 cells analyzed by wound healing assays. The migration of UM1 cells transfected with miR-221 inhibitor mimic plus si-NC set to 100%. (g) Representative images of UM1 invasion are shown. The invasion of UM1 cells was measured using a Matrigel invasion assay at 48 h after the transfection of miR-221 inhibitor mimic plus si-NC or miR-221 inhibitor mimic plus si-MBD2. (h) The average number of invading cells per field for the indicated experimental groups is shown. Data are presented as means ± SD. .

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