|
| Primers, PCR products, or bacteriocins | Nucleotide sequence (5′-3′) or description | Amplifications |
|
| Primers | | |
| S602-F | GCCATGAGCTCGAATTCTCGAGAAAAG | R-SRCAM602, R-E760, R-L1077 |
| S071-F | GTCCAGAGCTCGAATTCTCGAGAAAAG | R-SRCAM 602, R-E760, R-L1077, R-OR7 |
| SARP-R | AGGTACCATAAGTTGCGGCCGC | R-OR7 |
| ALFA-F | TACTATTGCCAGCATTGCTGC | pPICZαA amplification fragment including the cloned synthetic gene |
| 3AOX1-R | GCAAATGGCATTCTGACATCC | pPICZαA amplification fragment including the cloned synthetic gene |
| PCR products | | |
| R-SRCAM602 | 136-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic srcam602 gene with the P. pastoris codon usage | |
| R-OR7 | 181-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic or-7 gene with the P. pastoris codon usage | |
| R-E760 | 242-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic e-760 gene with the P. pastoris codon usage | |
| R-L1077 | 167-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic l-1077 gene with the P. pastoris codon usage | |
| Bacteriocins | | |
| BacSRCAM602 (amino acid sequence) | ATYYGNGLYCNKQKHYTWVDWNKASR
EIGKITVNGWVQH | |
| BacSRCAM602 (P. pastoris codon usage) | gctacttactacggtaacggtctttactgtaacaagcagaagcact
acacttgggttgactggaacaaggcttccagagagatcggtaag
atcactgttaacggttgggttcaaca | |
| BacOR-7 (amino acid sequence) | KTYYGTNGVHCTKNSLWGKVRLKNMK
YDQNTTYMGRLQDILLGWATGAFGKTFH |
| BacOR-7 (P. pastoris codon usage) | aagacttactacggaactaacggtgttcactgtactaagaattcctt
gtggggtaaggttagattgaagaacatgaagtacgaccagaaca
ctacttacatgggtagattgcaggacatcttgttgggttgggctact
ggtgctttcggtaagacttttcat |
| BacE-760 (amino acid sequence) | NRWYCNSAAGGVGGAAVCGLAGYVGE
AKENIAGEVRKGWGMAGGFTHNKACKS
FPGSGWASG |
| BacE-760 (P. pastoris codon usage) | aacagatggtactgtaactccgctgctggtggtgttggtggtgct
gctgtttgtggtttggctggttatgttggtgaggctaaagaaaacat
tgctggtgaggttagaaagggttggggtatggctggtggtttcac
tcataacaaggcttgtaagtccttcccaggttctggttgggcttctggt |
| BacL-1077 (amino acid sequence) | TNYGNGVGVPDAIMAGIIKLIFIFNIRQGY
NFGKKAT |
| BacL-1077 (P. pastoris codon usage) | actaactacggtaacggtgttggtgttccagacgctattatggctg
gtatcatcaagttgatcttcatcttcaacatcagacagggttacaac
ttcggtaagaaggctact |
|
