SimFuse: A Novel Fusion Simulator for RNA Sequencing (RNA-Seq) Data

Figure 2

SimFuse workflow. (a) Fusion free reads are extracted from the real data. (b) The expression quantification from real data and a genome reference are used to simulate fusion reads. (c) Fusion-free reads and simulated fusion reads are merged. (d) Running a fusion-detection algorithm on the simulation dataset generates results of detected fusions. Comparison of these results with the list of simulated fusions yields fusion-detection performance estimates (recall and precision) for the algorithm.