SimFuse: A Novel Fusion Simulator for RNA Sequencing (RNA-Seq) Data

Figure 3

Workflow of generating fusion supporting reads. An exon in gene and an exon in gene are randomly selected. Exon boundaries are used as the fusion boundaries. Spanning reads are randomly generated from within a prespecified range (read-length 3 by default) from the boundaries. To generate a splitting read, two fragments from the two genes and are generated, with their sum length being equal to the required read-length. A spanning read is randomly generated from either of the two exons to match the splitting read into a splitting read pair.