Optimizing Perfusion-Decellularization Methods of Porcine Livers for Clinical-Scale Whole-Organ Bioengineering

Figure 3

Retention of EMC components by decellularized liver scaffolds after three different SDS-based protocols. (a) Immunohistochemical stain (left 4 panels: collagen I, collagen IV, laminin, and fibronectin) and special stain (right 4 panels: AB staining, Masson’s trichrome staining, elastic fiber staining, and reticular fiber staining) of the three types of decellularized liver matrix. Scale bars: 100 μm. (b) The content of GAG in three group scaffolds. (c) The content of collagen in three group scaffolds. Normal liver was included as a control. versus T-SDS group.

(a)

(b)

(c)