Optimizing Perfusion-Decellularization Methods of Porcine Livers for Clinical-Scale Whole-Organ Bioengineering

Table 1

RT-PCR primer sequences.

Gene	Forward primers	Reverse primers
Alb	GGCACCAAGTGTTGTACCCT	AGCACACACAGACGGTTCAG
Cyp1a1	AGCTAATCAAAGAGCACTACAGG	CCTTATCATCTGAGAGCTGG
Cyp1a2	GAGAAGGTGATGCTCTTCGG	ATGCAGGAGGATGGCTAAGA
HNF4	CCTTGGACCCAGCCTACA	GCTTGAGGCTCCGTAGTGT
HNF6	CCTGGAGCAAACTCAAGTCC	CCGTGTTCTTGCTCTTTCC
Otc	TGAGGATCCTGCTCAACAAG	ACGGCCTTTCAGCTGTACTT
NAGS	CCGTTCGGTGCTTCTAGACT	CAGGTTCACATTGCTCAGGA
Arg1	CAACACTCCGCTGACAACC	CAGATATGCAGGGGGTCAC
Asl	TCAACAGTATGGATGCCACC	CAAAGTTGAATTCCTTGGTACC
Ass	CCAGGAAGAAGGCACTGAAG	GCCTAGGAGATAGCGGTCCT
Cps1	ACATTGGCTGCAGAATACCC	ACAGCCCAGCACCATTATTC