Research Article
Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition
Table 1
Strains, plasmids, and primers used in this study.
| | Description | Reference |
| E. coli strains | | | BW25113 | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | [11] | BWa | BW25113 ΔackA-pta | [11] | BWap | BW25113 ΔackA-pta, ΔpoxB | [11] | BWapl | BW25113 ΔackA-pta, ΔpoxB, ΔldhA | [11] | BWapld | BW25113 ΔackA-pta, ΔpoxB, ΔldhA, ΔadhE | [11] | BWapldf | BW25113 ΔackA-pta, ΔpoxB, ΔldhA, ΔadhE, ΔpflB | [11] | Plasmids | | | pWYC09 | pBluescript II SK− derivatives containing phaCAB from R. eutropha with promoter PadhE, AmpR | [10] | pBBR1MCS-2 | Broad-host-range plasmid, KanR | [10] | pMCS2pdc | Promoter Ppdc inserted into pBBR1MCS-2 | This study | pMCS2pflB | pflB inserted into pMCS2pdc | This study | Primers | | | pdcF | 5′-ATACTCGAGTTACGCTCATGATCGCGGCATGTC | pdcR | 5′-CCCCATATGTTACTCCATATATTCAAAAC | plfF | 5′-GCTAGGCATATGTCCGAGCTTAATGAAAA | plfR | 5′-CCGAATTCTTACATAGATTGAGTGAAGGT |
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All oligonucleotides were synthesized by AuGCT Biotechnology (Beijing, China). Restriction endonuclease digestion sites were underlined.
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