Pigment Epithelium-Derived Factor Induces Endothelial Barrier Dysfunction via p38/MAPK Phosphorylation

Figure 4

MTs disassembly and F-actin rearrangement were involved in PEDF-induced endothelial barrier dysfunction. (a) Endothelial cells were incubated under the conditions described in Figure 3(a). All the cells were stained for a-tubulin (red) and the nucleus was stained for DAPI (blue). Representative images show the MTs organization. The boxed areas are shown at higher magnification in the inserts to illustrate details of MTs. Scale bars = 10 μm. (b) Endothelial cells were stained for F-actin (red). White and blue arrows indicate central and peripheral F-actin fibers in cells, respectively. Scale bars = 10 μm.

(a)

(b)