Research Article
Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress
Table 1
The sequences and use of the primers.
| | Primer name | Sequences () | Primer use |
| | GPATF1 | ATATTGC(T/A)G(A/C)AGGAATGGA(G/A)GA | For middle fragments | | GPATF2 | AATGGA(G/A)GA(A/G)(C/A)TGTAT(C/T)AGAA(T/C)TA | For middle fragments | | GPATR1 | GGAGG(G/A)GGCAT(A/G)AT(A/G)TCAT | For middle fragments | | GPATR2 | G(A/C)CCAGG(G/A)ACACCAG(C/A)ATGTT | For middle fragments | | LpGPAT F1 | TAAGCCGCTGGTAGATTTCA | For 3′RACE of LpGPAT | | LpGPAT F2 | TCTCAAACCATCAGACGGAAGC | For 3′RACE of LpGPAT | | LpGPAT R1 | AAGTGTGATGTTCCCAATGTATGA | For 5′RACE of LpGPAT | | LpiGPAT R2 | GTGGCCCTGCTTGAGTTTCT | For 5′RACE of LpGPAT | | LpGPAT R3 | TCTGCTTCCGTCTGATGGTTTGA | For 5′RACE of LpiGPAT | | LpGPAT R4 | CAAAGAAGATTCCTTCCCATACTG | For 5′RACE of LpGPAT | | EGPATF | GCAGGGGATAGGGTTATTACAGA | For expression of LpGPAT | | EGPATF | AGGATTCTCAATGGCAGCAGTA | For expression of LpGPAT | | actinF | TCCTCTTCCAGCCTTCTTT | For control gene | | actinR | TTCCTTGCTCATACGGTCA | For control gene | | ActinF | GTCCATCCATCGTCCACAG | For real-time quantitative PCR | | ActinR | CCTCAACAAGCCACCTACC | For real-time quantitative PCR | | GPN1 | CTCCACCCCCAAAGGTAGAAA | For real-time quantitative PCR | | GPN2 | TGGCAGCAGTAACTTCAGCGA | For real-time quantitative PCR |
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