Mutagenic Potential ofBos taurus Papillomavirus Type 1 E6 Recombinant Protein: First Description

Table 2

Frequency of micronucleated lymphocytes. Number of micronuclei observed perslide (MN) and number of mononucleated (), binucleated (), and polynucleated () lymphocytes and anaphase bridges (AB) observed pergroup. Based on these values, the micronucleated formation frequency () and the cytokinesis-block proliferation index (CBPI) and the media () are shown.

Sample	MN				AB		CBPI
Negative control
01	7	954	22	24	3	0.3181	1.070
02	5	963	19	18	3	0.2631	1.055
03	6	949	22	29	0	0.2727	1.051
04	3	953	17	30	1	0.1764	1.077
05	7	960	22	18	5	0.3181	1.058
Total	28	4,779	102	119	12
Positive control (cyclophosphamide)
01	32	906	51	43	6	0.6274	1.137
02	31	873	62	65	8	0.5000	1.192
03	48	890	70	40	7	0.6857	1.150
04	54	903	62	35	14	0.8709	1.132
05	50	875	52	73	4	0.9615	1.198
Total	215	4,447	297	256	39
BPV-1 E6 recombinant oncoprotein
01	51	892	60	48	4	0.8500	1.156
02	50	894	74	32	9	0.6756	1.138
03	49	900	53	47	10	0.9245	1.147
04	40	901	41	58	10	0.9756	1.157
05	38	905	43	52	10	0.7307	1.147
Total	228	4,492	271	237	43