A Kinase Anchoring Protein 9 Is a Novel Myosin VI Binding Partner That Links Myosin VI with the PKA Pathway in Myogenic Cells

Figure 5

MVI depletion affects cAMP and PKA levels in C2C12 myoblasts. (a) The amount of cAMP (presented as pmol/mL) in untreated C2C12, scrambled and MVI-KD myoblasts was estimated as described in Section 2. (b) Probing the levels of total (upper panel) and phosphorylated (lower panel) PKA in untreated C2C12, scrambled and MVI-KD myoblasts by means of Western blot technique with the respective antibodies. Right panels: quantitative analysis of the PKA and phospho-PKA content with respect to the level ofβ-actin. The results in (a) and (b) are presented as a mean ± SD from two experiments (for (a) run in duplicate); .

(a)

(b)