Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines

Figure 7

Basal activities of AMPK, P42/44, P38 MAPK, and NFκB p65 in HepG2 cells grown in either 1 (Low) or 4,5 g/L (High) glucose serum-free media during 30 mn. Western blots were performed with the same protein extracts, and blots were hybridized first with antiphosphorylated kinase antibody (α-Phospho), stripped, and then hybridized with antibody raised against whole kinase form (α-). (a) Representative blots. (b) Ratio of electrophoretic bands detected by phosphoprotein versus whole protein antibodies (mean values ± SD). Asterix indicates significant difference in high versus low glucose treatment.Student’s -test -value, (3 independent experiments).

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