Research Article
Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress
Table 5
BestKeeper analysis results of the candidate reference genes.
(a) |
| Gene name |
SD [±CP] |
Coefficient of correlation () | value | All samples | 1 | 2 | All samples | 1 | 2 | All samples | 1 | 2 |
| 18SrRNA | 0.43 | 0.53 | 0.33 | 0.76 | 0.83 | 0.75 | 0.001 | 0.001 | 0.001 | EF1α | 1.00 | 1.12 | 0.94 | 0.82 | 0.79 | 0.85 | 0.001 | 0.001 | 0.001 | ACTIN | 0.61 | 0.68 | 0.57 | 0.52 | 0.75 | 0.26 | 0.004 | 0.001 | 0.355 | TUA | 1.51 | 0.88 | 2.08 | 0.72 | 0.50 | 0.88 | 0.001 | 0.056 | 0.001 |
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|
(b) |
|
CV [%CP] |
Min [-fold] |
Max [-fold] |
SD [-fold] | All sample | 1 | 2 | All sample | 1 | 2 | All sample | 1 | 2 | All sample | 1 | 2 |
| 3.41 | 4.14 | 2.63 | | | | 2.85 | 2.60 | 1.36 | 1.35 | 1.45 | 1.26 | 3.56 | 3.94 | 3.37 | | | | 7.92 | 6.85 | 3.93 | 2.00 | 2.17 | 1.92 | 2.35 | 3.54 | 2.20 | | | | 3.67 | 3.13 | 1.96 | 1.53 | 1.60 | 1.48 | 6.03 | 2.58 | 8.28 | | | | 7.59 | 3.22 | 7.16 | 2.85 | 1.80 | 4.24 |
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1 stands for the different time period treatment and 2 stands for the different concentration treatment.
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