The Prevalence of Helicobacter pylori Virulence Factors in Bhutan, Vietnam, and Myanmar Is Related to Gastric Cancer Incidence

Table 1

Primer sequences.

Genes	Primer sequences (5′→3′)	PCR product (bp)	PCR conditions
cagA	ACC CTA GTC GGT AAT GGG	521	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)
	GCT TTA GCT TCT GAY ACY GC
vacA
s1/s2	ATG GAA ATA CAA CAA ACA CAC	259/268	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)
	CTG CTT GAA TGC GCC AAA C
m1/m2	CAA TCT GTC CAA TCA AGC GAG	567/642
	GCG TCA AAA TAA TTC CAA GG
jhp0562/β-(1,3)galT	TGA AAA GCC CTT TTG ATT TTG	301/602	95°C, 30 s; 56°C, 30 s; 72°C, 1 s (35 cycles)
	GCT GTA GTG GCC ACA TAC ACG

Y = C + T.