Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 1/2 and Hepatitis C Virus Using Sol-Gel Based Microarray

Figure 3

Specifying the size and position of the spots in the scanned image. There are 9 spots, including triplets for HIV 1/2 (S1, S2, and S3), HCV (S4, S5, and S6), and 3 positioning markers. Measurement of the signal intensity of the specified spots (arrow, spot analysis diameter) and background (arrow, background diameter) are shown. Upper spots (S1–S3) consisted of an HIV recombinant antigen mixture containing p24, gp36, gp41 (M type, O type), and gp120. Lower spots (S4-S5) consisted of a HCV recombinant antigen mixture containing Core, NS3, NS4, and NS5.