Cross-Platform Evaluation of Commercial Real-Time SYBR Green RT-PCR Kits for Sensitive and Rapid Detection of European Bat Lyssavirus Type 1
Table 4
Detection limit of PCR (LOD 95%) for two-step and one-step SYBR Green RT-PCR.
Two-step RT-qPCR
IScript RT + qPCR SsoFast BioRad
QuantiTect RT + qPCR Quantitect QIAGEN
Maxima RT + qPCR Maxima FERMENTAS
SS Vilo RT + qPCR Express INVITROGEN
qScript RT + qPCR Perfecta QUANTA
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
110
55
171
86
78
39
41
21
88
44
88
44
160
80
76
38
45
22
67
34
One-step qRT-PCR
Quantitect-1 step QIAGEN
Rotor Gene-1 step QIAGEN
Verso-1 step FERMENTAS
SSIII Platinium-1 step INVITROGEN
qScript-1 step QUANTA
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
Number of copies/sample
Number of copies/rxn
20
40
497
994
127
254
321
641
406
812
22
44
15
30
101
202
50
100
981
1962
All LOD 95% results were carried out using Probit model. All PCR comparisons were run on the Rotor Gene Q MDx thermocycler (in italic font) and on the Mx3005P thermocycler (in bold).
