Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods

Table 3

Recovery of sesame form peanut butter spiked with tahini using different ELISA methods.

Spike level	Current method		Neogen-Tepnel^1		ELISA Systems^2
	Sesame (ppm)	Recovery (%)	Sesame (ppm)	Recovery (%)	Sesame (ppm)	Recovery (%)
0	—	n.a.	—	n.a.	—	n.a.
1	0.71	71	2.53	253	0.09	9
5	3.84	77	12.4	248	0.32	6
10	7.51	75	24.1	241	0.53	5
50	43.2	87	119	238	2.09	4
100	104	104	214	214	4.11	4

1: LLOQ given by manufacturer is 6 ppm. Values below 6 ppm may be therefore imprecise.
2: LLOQ given by manufacturer is 0.5 ppm. Values below 0.5 ppm may be therefore imprecise.
n.a.: not applicable.