Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors

<div>The optimization of avidin concentration for coating the PCR tubes. Avidin was diluted in PBS buffer to a final concentration ranging from 0.1, 1, 10, 50, and 100 <i>μ</i>g/mL, respectively. Biotin-HRP was added to monitor the amount of avidin in the PCR tubes. H<sub>2</sub>O<sub>2</sub> substrate was used and 1 N HCl was used to stop and read the optimum density (O.D.) at 450 nm.</div>

BioMed Research International

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Figure 3

Figure 3: Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors 