Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors

Figure 6

Percentage of IN activity relative to control. The reactions were performed from three independent experiments, each performed in triplicate. The measurements of IN activity were normalized by setting the competitor/inhibitors-free control (processed) and the IN-free control (unprocessed) as 100% and 0%, respectively. Percentage of IN activity was calculated by comparing the percent of IN activity relative to the competitor/inhibitors-free control.