Effect and Mechanism of Total Flavonoids Extracted from Cotinus coggygria against Glioblastoma Cancer In Vitro and In Vivo

Figure 4

The percentage of apoptosis and necrosis in glioblastoma cells induced by CCF. Glioblastoma cells were treated with the indicated concentration of CCF for 24 h. (a) CCF-induced, dose-dependent apoptosis and necrosis occurred in U87, U251, and DBTRG-05MG glioblastoma cells. The cells were stained with Annexin V-FITC and analyzed by flow cytometry. (b) Dose-dependent apoptosis and necrosis of the different glioblastoma cells were induced by CCF for 24 h. Cell death was measured as the percentage of PI-positive cells using flow cytometry. The cell death observed in the treated cells was significantly different from the control. The values of cell death (apoptosis and necrosis) reported represent values of the mean ± SD of three separate experiments. , , and , compared with the control cells.

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