“Adherent” versus Other Isolation Strategies for Expanding Purified, Potent, and Activated Human NK Cells for Cancer Immunotherapy

Table 1

Cell count, viability, phenotype distribution, and fold expansion of “adherent” enriched and expanded cells from PBMCs of (a) cancer patients and (b) healthy donors.

(a)

Donor	PBMCs			Enriched and expanded cells on day 7		Enriched and expanded cells on day 14
	Viable cell count	Percent viability	Percent NK cells (CD3^−CD56^+)	Viable cell count	Percent viability	Total viable cell count	Viable NK cell count	Percent NK cells (CD3^−CD56^+)	Percent T cells (CD3^+CD56^−)	Percent NK-T cells (CD3^+CD56^+)	Percent viability
SB	20.0 × 10^6	82	30.5	14.0 × 10^6	88	72.0 × 10^6	63.3 × 10^6	87.9	9.2	1.5
CH	32.0 × 10^6	87	5.4	9.9 × 10^6	78	108.0 × 10^6	69.3 × 10^6	64.2	22.1	12.6
TP	10.0 × 10^6	90	7.9	5.6 × 10^6	58	36.0 × 10^6	30.8 × 10^6	85.5	12.2	1.6
RS	24.0 × 10^6	84	8.8	12.0 × 10^6	93	136.0 × 10^6	111.1 × 10^6	81.7	15.8	1.5
JB	24.6 × 10^6	87	14.3	16.8 × 10^6	84	64.0 × 10^6	56.6 × 10^6	88.4	4.1	7.0
WN	25.0 × 10^6	92	25.4	9.4 × 10^6	89	70.0 × 10^6	66.7 × 10^6	95.3	3.2	1.0
GW	23.5 × 10^6	94	6.4	12.0 × 10^6	92	30.0 × 10^6	27.2 × 10^6	90.6	6.9	0.5
MT	29.5 × 10^6	91	14.3	22.0 × 10^6	82	54.0 × 10^6	47.0 × 10^6	87.0	5.5	6.4
LS	25.0 × 10^6	87	4.0	13.0 × 10^6	92	40.0 × 10^6	39.2 × 10^6	98.0	0.8	1.0

(b)

Donor	PBMCs			Enriched and expanded cells on day 7		Enriched and expanded cells on day 14
	Viable cell count	Percent viability	Percent NK cells (CD3^−CD56^+)	Viable cell count	Percent viability	Total viable cell count	Viable NK cell count	Percent NK cells (CD3^−CD56^+)	Percent T cells (CD3^+CD56^−)	Percent NK-T cells (CD3^+CD56^+)	Percent viability
2	26.4 × 10^6	80	13.1	42.0 × 10^6	79	216.0 × 10^6	203.5 × 10^6	94.2	4.2	0.8
JL	27.0 × 10^6	84	17.2	19.0 × 10^6	94	180.0 × 10^6	152.6 × 10^6	84.8	8.1	6.1
4	28.0 × 10^6	77	5.7	50.0 × 10^6	95	409.2 × 10^6	285.6 × 10^6	69.8	14.5	15.4
AS	20.0 × 10^6	79	17.0	17.4 × 10^6	93	320.0 × 10^6	304.6 × 10^6	95.2	1.3	2.8
SS	25.0 × 10^6	86	15.7	21.0 × 10^6	93	201.6 × 10^6	197.4 × 10^6	97.9	0.8	0.5
^*SS	40.0 × 10^6	85	13.9	48.0 × 10^6	95	580.0 × 10^6	573.0 × 10^6	98.8	0.6	0.4
21	26.0 × 10^6	70	19.8	19.2 × 10^6	85	248.0 × 10^6	235.4 × 10^6	94.9	2.5	2.4
^*21	96.0 × 10^6	91	19.8	75.0 × 10^6	90	451.1 × 10^6	415.0 × 10^6	92.0	2.6	4.7

Large-scale expansion.
Bold numbers represent low, medium and high percent expanded NK cells with respective presence of T cells and NK-T cells.