Review Article
“Adherent” versus Other Isolation Strategies for Expanding Purified, Potent, and Activated Human NK Cells for Cancer Immunotherapy
Table 3
Change in the percent and MFI of CD62L on CD3−CD56+ cells from healthy donor PBMCs after enrichment and expansion. Analysis of CD62L expression on CD3−CD56+ cells before and after enrichment and expansion. PBMC populations and two-week expanded cells were stained with Pacific Blue conjugated anti-CD3 (Clone HIT3a, Biolegend), Phycoerythrin-Cyanine 7 (PE/Cy7) conjugated anti-CD56 (Clone MEM-188, Biolegend), and Allophycocyanin (APC) conjugated anti-CD62L (Clone P3.6.2.8.1, eBioscience) antibodies and fixed in 2% paraformaldehyde. For all flow cytometry experiments, appropriate IgG isotype controls were used to assess nonspecific staining. Cells were analyzed using a BD LSRII flow cytometer (BD Biosciences) and the data was analyzed using FlowJo Flow Cytometry Analysis Software (TreeStar Inc.).
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Large-scale expansion. |