Research Article

Lipid Emulsion Attenuates Acetylcholine-Induced Relaxation in Isolated Rat Aorta

Figure 6

Effect of Lipofundin MCT/LCT (0.1 and 0.2%, ) on the phosphorylation of endothelial nitric oxide synthase (eNOS) in human umbilical vein endothelial cells (HUVECs). HUVECs were treated with 10−5 M acetylcholine alone for 4 min or 10−5 M acetylcholine for 4 min after pretreatment with Lipofundin MCT/LCT (0.1 and 0.2%) for 1 hour. Data are the mean ± SD. indicates the number of independent experiments. versus control. versus 10−5 M acetylcholine alone. t-eNOS: total eNOS; p-eNOS: phosphorylated eNOS.