Research Article
Lipid Emulsion Attenuates Acetylcholine-Induced Relaxation in Isolated Rat Aorta
Figure 7
Effect of Lipofundin MCT/LCT (0.2%) alone and combined treatment with L-arginine (10−4 M) and Lipofundin MCT/LCT (0.2%) on the phosphorylation of endothelial nitric oxide synthase (eNOS) in human umbilical vein endothelial cells (HUVECs). HUVECs were treated with 10−5 M acetylcholine alone for 4 min, 10−5 M acetylcholine for 4 min after pretreatment with Lipofundin MCT/LCT (0.2%) for 1 hour, or 10−5 M acetylcholine for 4 min after treatment with Lipofundin MCT/LCT (0.2%) for 1 hour following pretreatment with 10−4 M L-arginine for 10 min. Data () are the mean ± SD. indicates the number of independent experiments. versus control. versus 10−5 M acetylcholine alone. versus Lipofundin MCT/LCT (0.2%) plus acetylcholine (10−4 M). t-eNOS: total eNOS; p-eNOS: phosphorylated eNOS.