Research Article
Extraction Optimization for Obtaining Artemisia capillaris Extract with High Anti-Inflammatory Activity in RAW 264.7 Macrophage Cells
Table 1
The central composite experimental design and experimental data for the optimization of conditions for obtaining Artemisia capillaris Thunb. extracts.
| Run | Independent variables1 | Response variables2 | (°C) | (%) | (h) | (%) | (%) | (%) |
| 1 | 42 (−1) | 20 (−1) | 3 (−1) | 73.38 ± 0.21 | 79.18 ± 1.19 | 87.34 ± 2.04 | 2 | 78 (1) | 20 (−1) | 3 (−1) | 78.81 ± 0.15 | 83.32 ± 0.24 | 84.98 ± 4.38 | 3 | 42 (−1) | 80 (1) | 3 (−1) | 86.69 ± 0.13 | 87.21 ± 0.33 | 80.36 ± 0.52 | 4 | 78 (1) | 80 (1) | 3 (−1) | 67.11 ± 0.23 | 69.34 ± 0.32 | 81.46 ± 0.89 | 5 | 42 (−1) | 20 (−1) | 9 (1) | 74.38 ± 0.58 | 69.55 ± 1.06 | 85.98 ± 1.19 | 6 | 78 (1) | 20 (−1) | 9 (1) | 85.69 ± 0.79 | 85.08 ± 0.88 | 82.56 ± 1.34 | 7 | 42 (−1) | 80 (1) | 9 (1) | 68.32 ± 0.25 | 69.50 ± 0.24 | 82.88 ± 1.34 | 8 | 78 (1) | 80 (1) | 9 (1) | 73.38 ± 0.14 | 72.50 ± 0.16 | 86.92 ± 2.15 | 9 | 30 (−1.682) | 50 (0) | 6 (0) | 75.15 ± 0.42 | 78.66 ± 0.24 | 89.89 ± 0.41 | 10 | 90 (1.682) | 50 (0) | 6 (0) | 63.41 ± 0.11 | 63.18 ± 0.31 | 88.13 ± 2.75 | 11 | 60 (0) | 0 (−1.682) | 6 (0) | 71.65 ± 0.52 | 80.68 ± 0.24 | 85.24 ± 0.37 | 12 | 60 (0) | 100 (1.682) | 6 (0) | 75.15 ± 0.81 | 83.84 ± 0.16 | 85.50 ± 1.41 | 13 | 60 (0) | 50 (0) | 1 (−1.682) | 69.01 ± 0.19 | 75.92 ± 0.27 | 80.28 ± 1.30 | 14 | 60 (0) | 50 (0) | 11 (1.682) | 66.71 ± 0.14 | 71.36 ± 0.32 | 88.00 ± 0.26 | 15 | 60 (0) | 50 (0) | 6 (0) | 53.21 ± 0.21 | 56.50 ± 0.98 | 90.47 ± 1.26 | 16 | 60 (0) | 50 (0) | 6 (0) | 54.14 ± 0.47 | 56.03 ± 1.79 | 91.20 ± 1.15 | 17 | 60 (0) | 50 (0) | 6 (0) | 54.36 ± 0.23 | 56.03 ± 0.86 | 90.75 ± 1.89 | 18 | 60 (0) | 50 (0) | 6 (0) | 54.75 ± 0.15 | 57.12 ± 0.28 | 90.13 ± 0.74 | 19 | 60 (0) | 50 (0) | 6 (0) | 53.97 ± 0.50 | 56.65 ± 0.91 | 91.75 ± 1.71 | 20 | 60 (0) | 50 (0) | 6 (0) | 54.36 ± 0.14 | 55.87 ± 1.07 | 90.13 ± 2.34 |
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Independent variables: , extraction temperature; , ethanol concentration; , extraction time. 2Response variables: , LPS-induced PGE2 production; , LPS-induced NO production; , cytotoxicity.
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