A New Methodology for Evaluation of Nematode Viability

Figure 2

Analysis of viability using fluorimetric markers. (a) Microplate with serial dilution of C. elegans L₃ (first point 2,000 to 62). Rows A–D dead larvae; E–H represent viable (control) larvae. Correlation between the number of dead larvae with 50% methanol and the fluorescence intensity of the pixels with Sytox (b) and propidium Iodide (c). Comparison of densitometry (number of pixels) between Sytox and propidium iodide markers (d). Significant differences () were only detected when 1000 and 500 larvae were tested.

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