454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature

Table 3

Values for individuals computed, original species richness (est), interpolated species richness (int), and extrapolated species richness (ext).

	Temperature	Individuals (computed)	(est)	(int)	(ext)
Lab MBR (T2)	44°C	11646	42	34.8	42.99
	45°C	16895	148	85.6	148.4
	46°C	17336	117	67	117
	47°C	3162	78	76.8	85.67
	48°C	5883	100	79.2	108.9
	49°C	3024	105	105	117.3
Low Temperature CANON (TC)	44°C	11438	61	30.1	38.65
	45°C	14362	179	100.3	179
	46°C	9545	155	94.4	163.1
	47°C	4145	119	99.5	131.6
	48°C	5068	133	99.3	147.9
	49°C	2394	87	87	95.53
Apeldoorn DEMON (A)	44°C	9226	78	29.3	78.76
	45°C	9924	161.7	45.1	161.7
	46°C	654	73	55.6	79.11
	47°C	2192	141	66.5	156.9
	48°C	8206	258	68	265.2
	49°C	321	60	59.9	68.55
Olburgen CANON (N)	44°C	17193	87	41.9	87
	45°C	2893	198	134.2	218.3
	46°C	947	141	140.9	160.7
	47°C	3180	205	135.7	221.9
	48°C	2462	197	139.2	217.1
	49°C	1905	174	133.8	192.6
Rotterdam 2-stage anammox (R)	44°C	12977	81	43.2	81
	45°C	5183	211	147.3	228
	46°C	3879	188	143.5	205.2
	47°C	4120	198	151.6	212.3
	48°C	1679	143	143	159.6
	49°C	1791	145	142.3	159.7