Alleviation of Kainic Acid-Induced Brain Barrier Dysfunction by 4-O-Methylhonokiol in In Vitro and In Vivo Models

Figure 2

Changes and protective effects of MH, scavengers on the brain water content in KA-induced mice. (a) Male mice were grouped ( or 6/group). Seizures were induced by KA injection (10 mg/kg, i.p.); the mice in the saline group received an equal volume of 0.9% NaCl. Water contents were measured 0, 1, 3, and 48 h after KA or control injection. (b) Male mice were grouped ( or 6/group). Seizures were induced by KA injection (10–30 mg/kg, i.p.); the mice in the control group received an equal volume of 0.9% NaCl. (c) Male mice were grouped ( or 6/group) and pretreated with MH (5–20 mg/mouse, i.p.), trolox (50 mg/kg, i.p.), DMTU (50 mg/kg, i.p.), or NaCl (0.9%). Fifty minutes after MH or saline pretreatment, seizures in the KA and MH + KA groups were induced by KA injection (10 mg/kg, i.p.); the mice in the control group received an equal volume of 0.9% NaCl. The water contents of brain tissue was detected by measurement of the ratio of brain weight and body weight. The percentage of water content was calculated as [brain weight/bodyweight] × 100%. All weight data are presented as means ± SE. , versus control group. , versus KA group.

(a)

(b)

(c)