Alleviation of Kainic Acid-Induced Brain Barrier Dysfunction by 4-O-Methylhonokiol in In Vitro and In Vivo Models

Figure 3

Changes and ameliorative effect of MH, scavengers on the BBB permeability in KA-induced mice. (a) Male mice were grouped ( or 6/group). Seizures were induced by KA injection (10 mg/kg, i.p.); the mice in the control group received an equal volume of 0.9% NaCl. Permeability was measured colorimetrically 0, 1, 3, and 48 h after KA or control injection. (b) Male mice were grouped ( or 6/group). Seizures were induced by KA injection (10–30 mg/kg, i.p.); the mice in the control group received an equal volume of 0.9% NaCl. (c) Male mice were grouped ( or 6/group) and pretreated (i.p. injection) with MH (5–20 mg/kg, i.p.), trolox (50 mg/kg, i.p.), DMTU (50 mg/kg, i.p.), or NaCl (0.9%). Fifty minutes after MH or control pretreatment, seizures in the KA and MH + KA groups were induced by KA injection (10 mg/kg, i.p.); the mice in the control group received an equal volume of 0.9% NaCl. EBD (100 mg/kg, i.v) was injected 5 minutes prior to KA administration. Two days after the KA administration, dye in the brain tissue was extracted and determined colorimetrically. All data are presented as means ± SE. versus control group. , versus KA group.

(a)

(b)

(c)