Delay of Morphine Tolerance by Palmitoylethanolamide

Figure 3

Iba1-positive cell density in the dorsal horn of the spinal cord. 30 mg kg⁻¹ PEA s.c. and 10 mg kg⁻¹ morphine i.p. were administered daily and immunohistochemical analysis was performed on days 6 and 11; (a) representative images of merged Iba1-labeled microglia cells (red), plus DAPI-labeled cell nuclei (blue); scale bar: 50 μm. (b) Quantitative analysis of cellular density was performed evaluating 6 animals for each group. Each value represents the mean ± SEM of 6 rats per group, performed in 2 different experimental sets. ^* and ^** versus vehicle + vehicle; ^## versus vehicle + morphine.

(a)

(b)