Research Article

Delay of Morphine Tolerance by Palmitoylethanolamide

Figure 5

Colocalization of TNF-α and OX42 in the dorsal horn of the spinal cord. 30 mg kg−1 PEA s.c. and 10 mg kg−1 morphine i.p. were administered daily and immunohistochemical analysis was performed on days 6 and 11; (a) representative images of merged TNF-α (red), OX42 (green), and DAPI (blue) labeling; scale bar: 50 μm. (b) Quantitative analysis of the overlap coefficient for TNF-α and OX42 expression performed evaluating 6 animals for each group. Each value represents the mean ± SEM of 6 rats per group, performed in 2 different experimental sets.
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