Icariin Intervenes in Cardiac Inflammaging through Upregulation of SIRT6 Enzyme Activity and Inhibition of the NF-Kappa B Pathway

Figure 6

(a) Comparison of mRNA level of SIRT6 in different treatment groups. Young aortic ECs + PBS or + DMSO were controls. Various treatments of young aortic ECs are shown. After treatment with TNF-α, the mRNA expression level of SIRT6 was increased. Treatment with TNF-α and ICA resulted in a significant increase in SIRT6 mRNA relative to TNF-α treatment alone. TNF-α + ICA treated cells subsequently treated with PDTC or Nicotinamide or PDTC and Nicotinamide also showed increased SIRT6 mRNA levels; however there were no significant differences between these treatment groups and treatment with TNF-α + ICA. Note: TNF-α + ICA compared with TNF-α treated group, ; TNF-α + ICA + PDTC, TNF-α + ICA + Nicotinamide and TNF-α + ICA + PDTC(P) + Nicotinamide(N) compared with TNF-α + ICA group, . (b) Comparison of mRNA levels of TNF-α, ICAM-1, IL-2, and IL-6 in different treatment groups. Young aortic ECs treated with PBS or DMSO were the negative controls. Various treatments of young aortic ECs are shown. When given an exogenous TNF-α stimulus, mRNA levels of TNF-α, ICAM-1, IL-2, and IL-6 were significantly increased in all treatment groups. Treatments TNF-α and ICA resulted in a significant reduction in mRNA levels compared with TNF-α treatment alone. Treatment with ICA + PDTC further reduced the mRNA levels of the four inflammatory genes. Treatment with ICA + Nicotinamide partially reversed the effect of ICA, with significant increases in TNF-α and ICAM-1 mRNA levels. Treatment with ICA + PDTC + Nicotinamide significantly increased mRNA levels of the four inflammatory genes compared with the TNF-α + ICA + PDTC treated group and completely reversed the effect of ICA on ICAM-1 levels. Note: TNF-α + ICA compared with TNF-α treated group, or . TNF-α + ICA + Nicotinamide compared with TNF-α + ICA treated group, . TNF-α + ICA + PDTC(P) + Nicotinamide(N) compared with TNF-α + ICA + PDTC treated group or .

(a) mRNA levels of SIRT6 following TNF-α and ICA treatment

(b) mRNA expression levels of TNF-α, ICAM-1, IL-2, and IL-6