Screening Active Compounds from Garcinia Species Native to China Reveals Novel Compounds Targeting the STAT/JAK Signaling Pathway

Figure 2

The inhibition effect of the five chosen compounds (GB32, GB39, GB40, GB49, and GB63) on NCI-H1650 cells. (a) Inhibitory effects of compounds on the viability of NCI-H1650 cells assayed by trypan blue exclusion. Cells were treated for 24, 48, and 72 hr, and the viable cells were identified by trypan blue exclusion and counted. The IC50 values were calculated using XLfit software and a four-parameter model. NCI-H1650 cells were treated for 72 hr with compounds at concentrations ranging from 20 μM to 0.009 μM in serial 3-fold dilutions. (b) Cell colony formation curve after compound treatment measured using the Alamar Blue reagent from Invitrogen. The IC50 values were calculated using XLfit software and a four-parameter model.

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