Screening Active Compounds from Garcinia Species Native to China Reveals Novel Compounds Targeting the STAT/JAK Signaling Pathway

Figure 3

Caspase-3/7 induction and apoptosis induction by cell death detection in NCI-H1650 cells after compound treatment. (a) and (b) Caspase-3/7 activity induction was evaluated after 8 and 24 hr of treatment with the five compounds (GB32, GB39, GB40, GB49, and GB63) using Caspase-Glo 3/7 kit from Promega. Bars on the graph represent the mean fold induction relative to the DMSO control. (c) and (d) Apoptosis induction of cell lysate was assessed by oligonucleosomal fragmentation after 8-hour and 24-hour incubation with treatment compounds (GB32, GB39, and GB40). Data are shown relative to DMSO controls set at 1.0 ± SEM. (e) Apoptosis induction in NCI-H1650 cells by GB32, GB39, and GB40 was assessed ± the caspase inhibitor Z-VAD (10 μmol/L). The maximal apoptosis induction for each compound was chosen for the assessment of apoptosis reversal by Z-VAD. Data are shown relative to DMSO controls set at 1.0 ± SEM. (f) Compounds induced accumulation of G2 phase cells. NCI-H1650 cells were treated with 2.5 μM of each compound for 16 h. The cells were harvested, fixed in 70% EtOH, and stained with PI. The cell cycle was detected by FACS.

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