Effects of Two Fullerene Derivatives on Monocytes and Macrophages

Figure 8

Evaluations of ROS produced by PMA-activated macrophages with the CM-H2DCF-DA probe. MDM-LPS response of controls to the NADPH activator PMA is shown in (a). The kinetic ROS production of fullerene 1 (b) and fullerene 2 (c) pretreated cells (gray symbols) is compared to that of untreated control (black symbols), and all (untreated and treated) were stimulated with 0.1 μM PMA. Each point represents the mean value ± SEM of at least three independent determinations, obtained by the ratio of the fluorescence emitted by PMA stimulated cells/basal fluorescence, measured as MFI at each time of -axes. versus untreated controls, post-ANOVA Student-Newman-Keuls multiple comparison test.

(a)

(b)

(c)

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