Effects of Two Fullerene Derivatives on Monocytes and Macrophages

Table 3

Flow cytometric analysis of the fullerene derivatives interaction with monocytes and macrophages.

	Monocytes		MDM-LPS
	%	MFI	%	MFI
0 μM	0 ± 0.01	0 ± 0.01	0.16 ± 0.04	3.1 ± 0.4
5 μM fullerene 1-FITC	3.8 ± 0.3	3.0 ± 0.05	94.1 ± 0.06
10 μM fullerene 1-FITC	12.6 ± 1.2	3.2 ± 0.01	98.6 ± 0.19
5 μM fullerene 2-FITC	0.3 ± 0.03	3.1 ± 0.04	93.50 ± 0.23
10 μM fullerene 2-FITC	1.8 ± 0.19	3.4 ± 0.08	96.93 ± 0.36

Cells (1 × 10⁶/mL) were exposed for 24 h to 5–10 μM of fullerene derivatives: FITC tagged and subsequently recovered and run by flow cytometer. The percentage of positive cells and the mean fluorescent intensity of FL1 channel are reported. Each value is the mean ± SEM of triplicate samples. , versus untreated controls; versus the lower concentration (5 μM). Student-Newman-Keuls multiple comparisons test, ANOVA.