The Gastrointestinal Irritation of Polygala Saponins and Its Potential Mechanism In Vitro and In Vivo

Table 3

Influence of polygala saponins with different glycosyls: Onjisaponin B (OJB), tenuifolin (TEN), and senegenin (SNG) on isolated intestinal tension in rabbit in vitro.

Groups	Concentration (mg/L)	IIT (g)	CRIT (%)	Amplitude (g)
Control	—	0.04 ± 0.29	−0.53 ± 8.21	1.96 ± 0.55
SNG	20	−0.12 ± 0.26	−0.14 ± 12.98	1.52 ± 0.31
	40	−0.02 ± 0.37	1.60 ± 13.75	2.11 ± 0.43
	80	0.27 ± 0.49	8.31 ± 16.14	2.02 ± 0.93
TEN	20	0.09 ± 0.80	−3.06 ± 30.22	2.03 ± 0.84
	40	0.15 ± 0.30	−3.74 ± 11.36	1.87 ± 0.50
	80	0.57 ± 0.53^a	18.28 ± 26.44	2.11 ± 0.44
OJB	20	0.25 ± 0.42	12.84 ± 15.34^a	2.38 ± 0.56
	40	0.49 ± 0.28^2a	20.49 ± 16.61^2a	2.25 ± 0.31
	80	1.04 ± 0.62^3a	48.13 ± 34.39^3a	2.36 ± 0.71
PTS	20	−0.03 ± 0.52	−2.18 ± 18.07	1.82 ± 0.27
	40	0.26 ± 0.51	8.96 ± 16.54	2.33 ± 0.54
	80	0.58 ± 0.47^2a	19.2 ± 8.47^3a	1.90 ± 0.43

Mark: the solution was prepared and diluted to different concentrations (8, 4, and 2 mg/mL) for isolated gut motility experiment; 200 uL was added into per 20 mL Tyrode’s solution, measuring the average tension 3 min before and after the administration by the method of interval measurement. ( = 8, mean ± S.E.M.). ^a < 0.05; ^2a < 0.01; ^3a < 0.01 versus control. Increasing of intestinal tension (IIT) = Mean of   −  Mean of ;
Changing ratio of intestinal tension (CRIT) = (Mean of   −  Mean of )/Mean of   ×  100%.