Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles

Figure 1

Immunofluorescence staining of CYP17A1 and FSHR in theca cells. Scale bar = 100 μm. The purified cells were examined with immunofluorescence method after 24 h. By a series of titration experiments, the density of TCs was adjusted to approximately 70% confluence. FITC (green) staining CYP17A1 indicated the TCs origin. Hoechst staining (blue) indicated the location of cell nuclei. More than 90% of the cells were stained positive for CYP17A1, confirming the purity of TC cells population. Weak staining with FSHR antibody indicated that these TCs cultures were largely free of GCs contamination.